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abc hrp complex  (Vector Laboratories)


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    Structured Review

    Vector Laboratories abc hrp complex
    Abc Hrp Complex, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 18003 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vectastain+abc+kit/VECTASTAIN+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pmc13059316-74-29-31
    Average 96 stars, based on 18003 article reviews
    abc hrp complex - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Staining:

    Article Title: LRRK2 controls COX assembly through regulation of redox status of mitochondrial copper chaperones
    Article Snippet: .. Sections were stained with rabbit anti-tyrosine hydroxylase (TH) ( P40101 , Pel Freez) antibody and biotinylated goat anti-rabbit IgG secondary antibody, followed by streptavidin-conjugated horseradish peroxidase (Vectastain ABC kit, Vector Laboratories). .. Visualization of positive immunostaining was performed with 3,3′-diaminobenzidine (DAB, Sigma) after reaction with hydrogen peroxide (DAB kit, Vector Laboratories).

    Article Title: Early Binding of Anti-Amyloid Antibodies to CAA Drives Complement Activation, Inflammation and ARIA in Mice
    Article Snippet: The next day after 1x PBS washes, sections were incubated with secondary antibody, biotinylated Goat Anti-Rat IgG Antibody, Mouse Adsorbed (1:500, Vector Laboratories, #BA9401.5) followed by 1x PBS washes. .. Vectastain ABC kit (Vector Laboratories, #PK-6100) was used to label biotin with HRP as per the manufacturer’s instructions and visualized with a 3,3′-Diaminobenzidine (DAB) staining kit (Vector Laboratories, #SK-4100). .. Following Prussian blue staining as discussed below, sections were blocked with SuperBlock Blocking Buffer and overnight 4°C incubation with primary antibody against S97 Aβ (1:2000), washes with 1x PBS and 30 min secondary antibody incubation with Goat Anti-Rabbit IgG conjugated with Alkaline Phosphatase (1:100, SouthernBiotech, #4050-04), visualized with Vector Red substrate kit (Vector Laboratories, #SK-5100).

    Article Title: Lactate metabolism links reactive microglia, amyloid pathology, and Aβ dynamics.
    Article Snippet: .. The stain was developed using a Vectastain ABC kit (PK-6100, Vector Labs) and DAB reaction (ICN980681, Fisher Scientific). ..

    Incubation:

    Article Title: Interleukin-9 Regulates NF-kB-Mediated Activation of Astrocytes in Multiple Sclerosis Brain
    Article Snippet: .. Sections were then incubated with biotinylated donkey anti-mouse antibody (Jackson ImmunoResearch) for 160 minutes, followed by avidin biotin-peroxidase reactions using the Vectastain ABC kit (Vector Laboratories) and 3,3′-diaminobenzidine (Sigma-Aldrich) as a chromogen. ..

    Article Title: Comprehensive analysis of cytokines in depression: independent data from patient plasma and post-mortem ventromedial prefrontal cortex
    Article Snippet: Next, a 1:500 concentration of biotinylated goat anti-rabbit secondary antibody (Vector Laboratories, BA-1000) diluted in PBS with 0.2% Triton X-100 was used to incubate the vmPFC sections for 1 h at room temperature. .. Following this incubation, the samples were treated with the Vectastain ABC kit (Vector Laboratories, PK-4000) protocol for 30 min. .. The staining was then developed in DAB (Vector Laboratories, SK-4100) prepared in dH2O.

    Article Title: The intake of monosodium aspartate attenuates aggression induced by post-weaning social isolation in an ADHD rat model
    Article Snippet: On the second day, the sections were rinsed in PBST containing 1 % horse serum and incubated for 120 min at room temperature (RT) with biotinylated anti-mouse IgG (Vector Laboratories, Inc., California, BA-2000, lot #ZB0622) diluted 1:200 in PBST with 1 % normal horse serum. .. Following PBS washes, the sections were incubated for 60 min at RT with an avidin-biotin complex (Vectastain ABC Kit, Vector Laboratories, Inc., USA, PK-4000, lot #ZK0713) diluted 9 μL/mL in PBST containing 1 % normal horse serum. ..

    Article Title: Voiding symptoms aggravate with decreasing stromal/epithelial ratio and increasing glandular-epithelial content in patients undergoing laser enucleation for benign prostatic hyperplasia, independently from prostate size
    Article Snippet: .. For detection of calponin and pan-cytokeratin, membranes were then incubated with secondary biotinylated horse anti-mouse IgG (BA-2000) (Vector Laboratories, Burlingame, CA, USA) diluted 1:1500 in PBS-T containing 5% milk powder, washed again with PBS-T (4 times, each time for 5 min), incubated with avidin and biotinylated horseradish peroxidase (HRP) from the “Vectastain ABC kit” (Vector Laboratories, Burlingame, CA, USA) both diluted 1:800 in PBS and washed again with PBS-T (4 times, each time for 5 min). .. For detection of β-actin, membranes were incubated with secondary goat anti mouse IgG, H&L Chain Specific Peroxidase Conjugate (401,215) (Sigma-Aldrich, Munich, Germany) diluted 1:3000 in PBS-T containing 5% milk powder, and washed again with PBS-T (4 times, each time for 5 min).

    Avidin-Biotin Assay:

    Article Title: Interleukin-9 Regulates NF-kB-Mediated Activation of Astrocytes in Multiple Sclerosis Brain
    Article Snippet: .. Sections were then incubated with biotinylated donkey anti-mouse antibody (Jackson ImmunoResearch) for 160 minutes, followed by avidin biotin-peroxidase reactions using the Vectastain ABC kit (Vector Laboratories) and 3,3′-diaminobenzidine (Sigma-Aldrich) as a chromogen. ..

    Article Title: The intake of monosodium aspartate attenuates aggression induced by post-weaning social isolation in an ADHD rat model
    Article Snippet: On the second day, the sections were rinsed in PBST containing 1 % horse serum and incubated for 120 min at room temperature (RT) with biotinylated anti-mouse IgG (Vector Laboratories, Inc., California, BA-2000, lot #ZB0622) diluted 1:200 in PBST with 1 % normal horse serum. .. Following PBS washes, the sections were incubated for 60 min at RT with an avidin-biotin complex (Vectastain ABC Kit, Vector Laboratories, Inc., USA, PK-4000, lot #ZK0713) diluted 9 μL/mL in PBST containing 1 % normal horse serum. ..

    Article Title: Voiding symptoms aggravate with decreasing stromal/epithelial ratio and increasing glandular-epithelial content in patients undergoing laser enucleation for benign prostatic hyperplasia, independently from prostate size
    Article Snippet: .. For detection of calponin and pan-cytokeratin, membranes were then incubated with secondary biotinylated horse anti-mouse IgG (BA-2000) (Vector Laboratories, Burlingame, CA, USA) diluted 1:1500 in PBS-T containing 5% milk powder, washed again with PBS-T (4 times, each time for 5 min), incubated with avidin and biotinylated horseradish peroxidase (HRP) from the “Vectastain ABC kit” (Vector Laboratories, Burlingame, CA, USA) both diluted 1:800 in PBS and washed again with PBS-T (4 times, each time for 5 min). .. For detection of β-actin, membranes were incubated with secondary goat anti mouse IgG, H&L Chain Specific Peroxidase Conjugate (401,215) (Sigma-Aldrich, Munich, Germany) diluted 1:3000 in PBS-T containing 5% milk powder, and washed again with PBS-T (4 times, each time for 5 min).

    Amplification:

    Article Title: Sequoia affects Drosophila central nervous system development by regulating axonal extension and guidance
    Article Snippet: We used BP102 antibody from DSHB and antibodies that recognize Futsch (22C10 - DSHB), Fasciclin II (1D4 - DSHB), Eve (3C10 - DSHB), FasIII (7G10 – DSHB), Sequoia [ ], GFP (Molecular Probes and Roche), ßGal (Cappel and Promega), and Cy2, Cy3 and Cy5-conjugated secondary antibodies (Jackson ImmunoResearch). .. For HRP histochemistry, the signal was amplified using the Vectastain-ABC kit (Vector Laboratories) when required. .. Photographs were taken using Nomarski optics in a Nikon Eclipse 800 microscope.



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